Key Idea #5 in the Living Environment section requires students to "explain the biochemical processes in living organisms and their importance in maintaining homeostasis" Specifically, this investigation had students closely examine how enzyme molecules "have specific shapes that influence both how they function and how they interact with other molecules." (Major Understanding 5.1g) This was done in the context of Performance Indicator 5.1 which states "Explain the basic biochemical processes in living organisms and their importance in maintaining dynamic equilibrium." Students also addressed Performance Indicator 5.2 which asks them to "Explain disease as a failure of homeostasis." They focused on the component of Major Understanding 5.2h which makes them aware of how disease may be caused as a result of poor nutrition.

This activity also involved students in MST Commencement Standards #1 and #2. Students had to "use mathematical analysis and scientific inquiry…to pose questions, seek answers, and develop solutions." They also had "to access, generate, process, and transfer information using appropriate technologies." Specifically, students had to demonstrate an ability to use information technology "to retrieve, process, and communicate information and as a tool to enhance learning." (Standard #2, Key Idea #1) Students were also required to "access, select, collate, and analyze information obtained from a wide range of sources such as research data bases, foundations, organizations, national libraries, and electronic communication networks, including the Internet." (3rd Performance Indicator for Key Idea #1). Based on their own original data (Standard #1 Key Ideas 1 and 2, Mathematical Analysis and Standard 3, Key Ideas 1 and 4) and their findings using information technology, students had to design and carryout a scientific experiment. (Standard #1, Key Ideas 1,2,3 Scientific Inquiry). As a result of doing the basic experiment, conducting a literature search, designing and performing their own experiment, students came to "understand the relationships and common themes that connect mathematics, science, and technology." (Standard #6). They also had the opportunity to "apply the knowledge and thinking skills of mathematics, science, and technology to address real-life problems and make informed decisions." (Standard #7)

Students should be aware of the importance of enzymes in digestion, and should have experience in designing graphs. It would also be helpful if they understood the concept of how to write up an experiment.